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in EBSD
explained:
Basics of EBSD
EBSD Experiments
Undertaking Experiments
Sample Preparation
Links to this section:
Sample Preparation Camera integration time Background removal Microscope conditions Sample tilt Resolution and accuracy Summary
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Undertaking EBSD experiments
Camera integration time and resolution
Because the luminous intensity of
the diffraction pattern on the phosphor screen is low, successive frames may
need to be integrated in the CCD camera to improve the signal to noise ratio
in the image. Using too short
an integration time on the CCD camera may give a poor image and in
this case, integrating for longer on the CCD will improve the visibility of
the diffraction pattern (Figure 1). The camera may be cooled to reduce
electronic noise in the CCD when used in this way. The yield of backscattered electrons increases with atomic number, so low atomic number
materials will require a longer integration time than higher atomic numbers.
The CCD camera resolution can
also effect the integration time required to collect a diffraction pattern.
Current CCD cameras can collect 12 bit images at a resolution of 1300 x 1024
pixels. Using “pixel binning”, neighbouring pixels in the CCD can be added
together to form a single pixel in the image. When pixels are binned in this
way, less integration time is required to achieve a given signal in the
image pixel because the detecting pixel area is larger. For example, if a
satisfactory diffraction pattern is obtained in 12 ms at 1300 x1024
resolution, a comparable pattern can be obtained in 3 ms at 650 x 512
resolution.
It is not essential to collect
high resolution diffraction patterns for crystal orientation mapping because
the Hough transform always operates on a 128 x128 resolution image. In
addition, the transform works well on noisy images (Figure 2) so some
experimentation is necessary to determine the optimum integration time
required when collecting maps.
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